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. Author manuscript; available in PMC: 2019 Dec 11.
Published in final edited form as: Biochemistry. 2019 Nov 26;58(49):4970–4982. doi: 10.1021/acs.biochem.9b00878

Figure 4.

Figure 4.

Titration of pyruvate onto (A) H49A, (B) H49N, (D) H299A, and (E) H299N monitored by CD under anaerobic conditions. Insets illustrate enzyme-subtracted spectra for H49A and H299A. Overlay of CD spectra of (C) H49A variants and the wild type or (F) H299 variants and the wild type in the presence of a saturating pyruvate concentration indicating a lower signal intensity for LThDP accumulation on all variants compared to the wild type and on Ala variants compared to Asn variants at the same position.