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. Author manuscript; available in PMC: 2019 Dec 11.
Published in final edited form as: Biochemistry. 2019 Nov 26;58(49):4970–4982. doi: 10.1021/acs.biochem.9b00878

Figure 7.

Figure 7.

Expanded limited trypsinolysis time course of (A) H49A and (B) H299N in the absence (left) and presence (right) of MAP. Full length DXP synthase (one asterisk), cleavage product 1 (44 kDa, two asterisks, cleavage at K227), and cleavage product 2 (34 kDa, three asterisks, cleavage at K313). Subtle accumulation over 10 min of the 44 kDa peptide is apparent when H49A and H299N are preincubated with a saturating MAP concentration in contrast to limited trypsinolysis of the wild type under the same conditions in which accumulation of the 44 kDa peptide is sustained over 150 min (Figure 6A).