Table 2.
Anaerobic Characterization of the Inhibition of H49 and H299 Variants by MAPa
| enzyme | KiMAP (μM) | IC50MAP at 2Kmpyruvate (μM) | IC50MAP at 10Kmpyruvate (μM) |
|---|---|---|---|
| wild type | (3.24 ± 0.02) × 100 | (1.00 ± 0.02) × 10 | (3.19 ± 0.04) × 10 |
| H49A | (1.25 ± 0.04) × 102 | (3.8 ± 0.1) × 102 | (1.06 ± 0.08) × 103 |
| H49N | (1.67 ± 0.08) × 102 | (5.2 ± 0.4) × 102 | (1.7 ± 0.1) × 103 |
| H299A | (1.08 ± 0.06) × 103 | (3.2 ± 0.1) × 103 | (1.0 ± 0.1) × 104 |
| H299N | (2.4 ± 0.2) × 102 | (7.8 ± 0.7) × 102 | (2.84 ± 0.05) × 103 |
a
The error represents the standard error, where n = 3.