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. Author manuscript; available in PMC: 2019 Dec 11.
Published in final edited form as: Biochemistry. 2019 Nov 26;58(49):4970–4982. doi: 10.1021/acs.biochem.9b00878

Table 3.

Rate Constants of Unfolding and Unfolding Half-Lives for the Four Peptides (51–58, 183–199, 188–199, and 278–298) Displaying EX1 Kinetics

51–58
 183–199
 188–199
 278–298
enzyme k (min−1) t1/2 (min) k (min−1) t1/2 (min) k (min−1) t1/2 (min) k (min−1) t1/2 (min)
wild type (3.3 ± 0.5) × 10−2 (2.1 ± 0.3) × 10 (2.4 ± 0.3) × 10−1 (3.0 ± 0.3) × 100 (1.0 ± 0.2) × 10−1 (6.2 ± 0.9) × 100 (1.9 ± 0.1) × 10−1 (3.7 ± 0.1) × 100
H49A (6 ± 3) × 100 (1.4 ± 0.6) × 10−1 (2.1 ± 0.6) × 100 (4 ± 1) × 10−1 (2.1 ± 0.4) × 100 (3.3 ± 0.6) × 10−1 (8 ± 3) × 100 (1.1 ± 0.5) × 10−1
H299N (1.9 ± 0.3) × 100 (3.8 ± 0.6) × 10−1 (2.7 ± 0.2) × 100 (2.6 ± 0.2) × 10−1 (4.3 ± 0.7) × 100 (1.7 ± 0.3) × 10−1 (4 ± 1) × 100 (1.7 ± 0.4) × 10−1