Figure 6. qRT-PCR validation of R2R3-MYB transcription factors in Medicago truncatula. All expressional levels of each R2R3-MYB genes were normalized with control expression value (group A) set as one. The groups B (cold stress), C (freezing stress), D (drought stress), E (salt stress), and F (ABA treatment) were compared with control group, and fold-changes were calculated. The fold-change data was used to create the plot, and the blue indicates RNA-seq results, while pink indicates qRT-PCR analysis results.