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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Fungal Genet Biol. 2019 Jul 26;132:103258. doi: 10.1016/j.fgb.2019.103258

Fig. 3. Biofilm-related C. neoformans cells display larger capsule and higher polysaccharide released than planktonic cells.

Fig. 3.

(A) Representative India ink images displaying the capsule size of cryptococcal planktonic and biofilm-related cells. Biofilm-derived fungal cells exhibited larger capsules than planktonic cells. The pictures were taken using a ×100-power field. Scale bar, 5-μm. (B) Capsule size measurements of planktonic and biofilm-derived C. neoformans cells were performed. The capsule size of 100 cells (each circle represents 1 macrophage) was measured. (C) GXM concentration in the supernatant of C. neoformans cultures of planktonic and biofilm-related cells. For B and C, bars represent the average and error bars indicate SDs. Asterisks denote P-value significance (* P<0.05 and *** P<0.0001) calculated using student’s t-test analysis. The legend in B applies to this graph. The experiments were performed twice, and similar results were obtained.