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. 2019 Dec 11;13(12):e0007819. doi: 10.1371/journal.pntd.0007819

Fig 1. Macrophage IL-4/IL-13 polarization alters susceptibility to rVSV/EBOV GP infection, but not rVSV/G.

Fig 1

A) Peritoneal macrophages isolated from C57BL/6 Ifnar-/- mice were treated with 20ng/ml IL-4 and IL-13 or left untreated for 24 hours. Cells were infected with rVSV/EBOV GP(MOI = 0.1) or rVSV/G (MOI = 0.033). As both viruses encode and express GFP, infection was quantified at 24 hours by detection of GFP+ cells by flow cytometry. Shown are the fold increases above unstimulated controls that are set as 1. B) Ex vivo matured human monocyte derived macrophages were treated with 20ng/ml of IL-4 and IL-13 for 24 hours prior to infection with rVSV/EBOV GP (MOI = 5) or WT VSV (MOI = 1). Infection was quantified at 24 hours by the number of GFP+ cells by flow cytometry and expressed relative to unstimulated controls. C) Peritoneal macrophages were harvested from female C57BL/6 mice, treated with 20ng/ml IL-4/IL-13 for 24 hours or left unstimulated, and infected with rVSV/EBOV GP(MOI = 1). Infection was quantified at 24 hours by flow cytometry and expressed relative to unstimulated controls. Data are shown as pooled results from two independent donors representative of many additional replicates. Data are expressed as mean ± S.D. Significance was determined by Student’s t-test comparing individual treatments to unstimulated controls, *p<0.05.