Key resources table.
| Reagents or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Cell culture | Matrigel | Corning | Cat # 354230 | |
| Cell culture | mTeSR 1 | STEMCELL Technologies |
Cat # 85850 | |
| Cell culture | Dorsomorphin | Tocris | Cat # 3093 | |
| Cell culture | A83-01 | Stemgent | Cat # 04–0014 | |
| Cell culture | PNU74654 | Tocris | Cat # 3534 | |
| Cell culture | DMEM/F12 | ThermoFisher | Cat # 10565018 | |
| Cell culture | N2 | ThermoFisher | Cat # 17502048 | |
| Cell culture | B27 | ThermoFisher | Cat # 17504044 | |
| Cell culture | FGF | R and D | Cat # 4114-TC | |
| Cell culture | p-ornithine | Millipore Sigma | Cat # 27378490 | |
| Cell culture | laminin | Trevigen | Cat # 340001001 | |
| Cell culture | Knockout Serum Replacement | ThermoFisher | Cat # 10828028 | |
| Cell culture | β-Mercaptoethanol | ThermoFisher | Cat # 21985023 | |
| Cell culture | BDNF | Peprotech | Cat # AF-450–02 | |
| Cell culture | GDNF | Peprotech | Cat # AF-450–10 | |
| Cell culture | ES-FBS | ThermoFisher | Cat # 16141079 | |
| Organoid culture | Cerebral Organoid Kit | STEMCELL Technologies | Cat # 08570 | |
| Critical commercial assay |
V-PLEX Plus Aβ Peptide Panel 1 (6E10) Kit |
Meso Scale Discovery |
Cat # K15200 | |
| Electrophysiology | K-Gluconate | Millipore Sigma | Cat # P1847 | |
| Electrophysiology | Cs-Gluconate | Hello Bio | Cat # HB4822 | |
| Electrophysiology | KCl | Millipore Sigma |
Cat # 60128 | |
| Electrophysiology | CsCl | Millipore Sigma |
Cat # C4036 | |
| Electrophysiology | MgCl2 | Fluka | Cat # 63020 | |
| Electrophysiology | HEPES | Millipore Sigma |
Cat # H4034 | |
| Electrophysiology | EGTA | Millipore Sigma |
Cat # 3889 | |
| Electrophysiology | Mg-ATP | Millipore Sigma |
Cat # A9187 | |
| Electrophysiology | HBSS | ThermoFisher | Cat # 14175079 | |
| Electrophysiology | CaCl2 | Millipore Sigma |
Cat # C5080 | |
| Electrophysiology | Glycine | Millipore Sigma |
Cat # G2879 | |
| Electrophysiology | Tetrodotoxin citrate |
Hello Bio | Cat # HB 1035 | |
| Electrophysiology | β-Secretase Inhibitor IV |
EMD Millipore | Cat # 565788 | |
| Electrophysiology | Compound E | EMD Millipore | Cat # 565790 | |
| Immunocytochemistry (ICC) | PFA | Alfa Aesar | Cat # 43368 | |
| ICC | BSA | Millipore Sigma |
Cat # A0336 | |
| ICC | Triton X-100 | Millipore Sigma |
Cat # T8532 | |
| ICC antibody | DAPI | Invitrogen | Cat # D1306; RRID:AB_2629482 |
1:500 |
| ICC antibody | Chicken polyclonal Anti-Tuj1 | Abcam | Cat # ab41489; RRID:AB_727049 |
1:200 |
| ICC antibody | Mouse monoclonal Anti-Synapsin 1 | Synaptic Systems |
Cat # 106 011; RRID:AB_2619772 |
1:500 |
| ICC antibody | Rabbit polyclonal Anti-Homer 1 |
Synaptic Systems |
Cat # 160 003; RRID:AB_887730 |
1:200 |
| ICC antibody | Mouse monoclonal Anti-VGAT |
Synaptic Systems |
Cat # 131 011; RRID:AB_887868 |
1:200 |
| ICC antibody | Rabbit polyclonal Anti-VGLUT1 | EMD Millipore | Cat # ABN1647; RRID:AB_2814811 |
1:500 |
| ICC antibody | Rabbit polyclonal Anti-GABA |
Sigma | Cat # A2052; RRID:AB_477652 |
1:100 |
| ICC antibody | Mouse monoclonal Anti-Parvalbumin | EMD Millipore | Cat # MAB1572; RRID:AB_2174013 |
1:100 |
| ICC antibody | Rabbit polyclonal Anti-FOXG1 |
Abcam | Cat # ab18259; RRID:AB_732415 |
1:250 |
| ICC antibody | Rat monoclonal Anti-CTIP2 | Abcam | Cat # ab18465; RRID:AB_2064130 |
1:200 |
| ICC antibody | Rabbit polyclonal Anti-TBR2 |
Abcam | Cat # ab23345; RRID:AB_778267 |
1:300 |
| ICC antibody | Mouse monoclonal Anti-Nestin |
Abcam | Cat # ab22035; RRID:AB_446723 |
1:200 |
| ICC antibody | Anti-Amyloid β 1–16 | Biolegend | Cat # 803001; RRID:AB_2564653 |
1:2000 |
| Immunoblot antibody | Mouse monoclonal Anti-VGAT |
Synaptic Systems |
Cat # 131011; RRID:AB_887868 |
1:500 |
| Immunoblot antibody | Rabbit polyclonal Anti-VGLUT1 |
Synaptic Systems | Cat # 135303; RRID:AB_887875 |
1:1000 |
| Software and Algorithms |
Clampex v10.6 (pClamp) | Molecular Devices |
RRID:SCR_011323 | |
| Software and Algorithms | Clampfit v10.6 (pClamp) | Molecular Devices |
RRID:SCR_011323 | |
| Software and Algorithms |
Mini Analysis | Synapstosoft |
http://www.synaptosoft.com/MiniAnalysis/; RRID:SCR_002184 |
|
| Software and Algorithms |
ImageJ | NIH |
https://imagej.nih.gov/ij/; RRID:SCR_003070 |
|
| Software and Algorithms |
Prism7 | GraphPad |
http://www.graphpad.com; RRID:SCR_002798 |