Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | HEK293 | ATCC | RRID:CVCL_0045 | |
| Cell line (Homo sapiens) | HAP1 | Horizon Discovery | Cat. #: C631 | |
| Cell line (Chlorocebus aethiops) | Cos1 | ATCC | RRID:CVCL_0223 | |
| Recombinant DNA reagent | pEGFP-C2-TPC2 (plasmid) |
Wang et al., 2012 | ||
| Recombinant DNA reagent | pEGFP-C2-TPC1 (plasmid) |
Wang et al., 2012 | ||
| Sequence-based reagent | TPC1-sgRNA | This paper | sgRNA | CTTGCAGTACTTCAGCACCC |
| Sequence-based reagent | TPC2-sgRNA | This paper | sgRNA | CCCCAGCGTCGGGCTGCTGC |
| Sequence-based reagent | TPC1-fw | This paper | PCR primers | ATGGCCCAGACATGTGACTC |
| Sequence-based reagent | TPC1-re | This paper | PCR primers | TGCCTGTCTCCATCCTCTCA |
| Sequence-based reagent | TPC2-fw | This paper | PCR primers | TGAGCTGAGCATGAGGCAAG |
| Sequence-based reagent | TPC2-re | This paper | PCR primers | AAAGGACAAGTGGCCCTGAG |
| Chemical compound, drug | Desipramin hydrochloride | Sigma | Cat. #: D3900 | |
| Chemical compound, drug | Carbamazepine | Sigma | Cat. #: C4024 | |
| Chemical compound, drug | monensin | Sigma | Cat. #: M5273 | |
| Chemical compound, drug | ionomycin | Sigma | Cat. #: I0634 | |
| Chemical compound, drug | Clomipramine | Cayman Chemical | Cat. #: 15884 | |
| Chemical compound, drug | Imipramine | Cayman Chemical | Cat. #: 15890 | |
| Chemical compound, drug | Amitriptyline | Cayman Chemical | Cat. #: 15881 | |
| Chemical compound, drug | Nortriptyline | Cayman Chemical | Cat. #: 15904 | |
| Chemical compound, drug | Phenothiazine | NCATS | CAS: 92-84-2 | |
| Chemical compound, drug | Triflupromazine | NCATS | CAS: 146-54-3 | |
| Chemical compound, drug | Chlorpromazine | Cayman Chemical | Cat. #: 16129 | |
| Chemical compound, drug | ML-SA1 | Princeton BioMolecular Research Inc | Cat. #: OSSK_389119 | |
| Chemical compound, drug | PI(3,5)P2 | Echelon Biosciences | Cat. #: P-3508 | |
| Chemical compound, drug | vacuolin-1 | Calbiochem | Cat. #: 673000 | |
| Software, algorithm | pClamp | pClamp | RRID:SCR_011323 |