Table 1. Summary of electrophysiology-based screening of TPC agonists.
Based on chemical structures, LyNa-VAs were divided into two groups: LyNa-VA1.x and LyNa-VA2.x. EC50 and the average TPC2 currents (ITPC2) were calculated based on 3–5 whole-cell or whole-endolysosome recordings (see individual data in Table 1—source data 1) for each LyNa-VA, respectively.
Table 1—source data 1. Electrophysiology-based screening of TPC agonists.
elife-51423-table1-data1.xlsx (19KB, xlsx)
| LyNa-VAs | Chemical name | Structure | EC50 (μM)* | ITPC2 (pA)** |
|---|---|---|---|---|
| LyNa-VA1.1 | Clomipramine |
 |
43 ± 2 | 945 ± 111 |
| LyNa-VA1.2 | Desipramine |
 |
87 ± 8 | 1120 ± 94 |
| LyNa-VA1.3 | Imipramine |  |
112 ± 1 | 433 ± 94 |
| LyNa-VA1.4 | Amitriptyline |
 |
102 ± 3 | 876 ± 196 |
| LyNa-VA1.5 | Nortriptyline |
 |
52 ± 10 | 1916 ± 361 |
| Carbamazepine |
 |
No activation | No activation | |
| LyNa-VA2.1 | Chlorpromazine |
 |
60 ± 2 | 1101 ± 508 |
| LyNa-VA2.2 | Triflupromazine |
 |
63 ± 2 | 984 ± 294 |
| Phenothiazine |
 |
No activation | No activation |
*Data were obtained from whole-cell recordings at −140 mV.
**Data were obtained from whole endolysosome recordings with 100 µM of LyNa-VAs at −120 mV.