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. 2019 Dec 11;5(12):eaax8898. doi: 10.1126/sciadv.aax8898

Fig. 7. Single deletion of Sox2 or Sox9 in gastric adenoma mice increases cancer severity, whereas DKO rescues tumor severity when compared to singly deleted counterparts.

Fig. 7

(A) SOX9 immunohistochemistry (top) and Alcian blue staining (bottom) show SOX9 overexpression at sites of adenomatous epithelium in early (8 weeks) and late (18 weeks) adenoma mice (n = 3 each). Scale bars, 100 μm. (B) Corresponding H&E (top) and Alcian blue (bottom) staining shows the aberrant changes in gastric epithelial structure and cell types in the various adenoma models (n ≥ 3 for Atp4bCre;R26NICD, Atp4bCre;R26NICD;Sox2flox/flox, Atp4bCre;R26NICD;Sox9flox/flox, and Atp4bCre;R26NICD;Sox2flox/flox;Sox9flox/flox mice). Scale bars, 100 μm. (C) SOX2 immunohistochemistry in Atp4bCre;R26NICD;Sox9flox/flox mice (top) and SOX9 immunohistochemistry in Atp4bCre;R26NICD;Sox2flox/flox mice (bottom) demonstrate reciprocal activation in the absence of either SOX TF (n = 3 each). Scale bars, 100 μm.