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. 2019 Dec 11;5(12):eaax9230. doi: 10.1126/sciadv.aax9230

Fig. 6. Expression of PTPRE-AS1 in human allergic asthmatic patients.

Fig. 6

(A) Analyses of the expression levels of PTPRE-AS1, PTPRE, and WDR5 in PBMCs from 40 allergic asthmatic patients and 42 healthy controls. (B) Analyses of the expression of the M2-associated genes in PBMCs from patients with allergic asthma and healthy controls. (C) Macrophages were induced using THP1 cells treated with phorbol 12-myristate 13-acetate (PMA) (500 ng/ml, 48 hours). Overexpression of PTPRE-AS1 in THP1 macrophages transfected with PTPRE-AS1 LV or NC LV. After 72 hours of transfection, M2-associated gene expression in IL-4–stimulated THP1 macrophages was quantified by RT-qPCR analysis. (D) Receiver operating curve (ROC) analysis of PTPRE-AS1, PTPRE, and WDR5 levels in the study population. (E) Correlation analysis of the expression of PTPRE-AS1 and PTPRE, or CD206 levels in patients with allergic asthmatic (Pearson’s correlation). (F) Schematic model of PTPRE-AS1 functions in IL-4–induced M2 macrophage activation. *P < 0.05; **P < 0.01; ***P < 0.001.