Skip to main content
. Author manuscript; available in PMC: 2019 Dec 11.
Published in final edited form as: Nature. 2019 Jun 5;570(7762):523–527. doi: 10.1038/s41586-019-1289-x

Extended Data Figure 2: Analysis of cell type-specific markers in organoids derived from different lines.

Extended Data Figure 2:

a, Expression of selected marker genes used in cell type identification. Violin plots show distribution of normalized expression in cells from CCA-aligned organoids at 3 months (n=9 organoids from 3 batches). CFuPNS: n=15,866 cells; CPNs, n=18,905 cells; Cycling, n=4,035 cells; Immature INs, n=353 cells; Immature PNs, n=6,727 cells; IPCs, 4,276 cells; oRG, n=5,436 cells; and RG, n=3,318 cells. Scale: normalized read counts. b, IHC for neuronal (MAP2), dorsal forebrain progenitor (EMX1 and PAX6), CFuPN (CTIP2), CPN (SATB2), radial glia (SOX2), and proliferation (Ki67) markers in PGP1 (b2), 11a, GM08330, and HUES66 organoids at 3 months. c, In situ RNA hybridization for the IPC (EOMES a.k.a. TBR2), Cajal-Retzius (Reelin), and post-mitotic PN (TBR1) markers in 3 month PGP1 (b2), 11a, GM08330, and HUES66 organoids. d, IHC for the forebrain progenitor (FOXG1), outer radial glia (HOPX), post-mitotic PN (TBR1), and IPC (TBR2) markers in PGP1 (b2) organoids at 3 and 6 months (scale bars for B, C, D: 50 μm). Abbreviations as in Figure 1.