Table 1. Thermodynamic parameters for taurine binding to TauA and mutants.
Kd (nM) | Stoichiometry | ΔH (kJ/mol) | ΔS (J/mol/K) | |
---|---|---|---|---|
WT | 1.6 | 0.768(±0.38%) | −61.04 (±0.08%) | −3.05 |
Gln30Ala | 3480 | 0.83(±6%) | −17.87 (±8%) | 3.72 |
Asp106Ala | NB | NB | NB | NB |
Thr132Ala | 20 620 | 0.8* | −28.76 (±8%) | −0.54 |
Asp205Ala | 22 880 | 0.8* | −34.83 (±2%) | −2.34 |
NB, no binding.
Stoichiometry fixed to 0.8 during data fitting.