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. 2019 Dec 5;10:2796. doi: 10.3389/fmicb.2019.02796

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Copyright © 2019 Pausan, Csorba, Singer, Till, Schöpf, Santigli, Klug, Högenauer, Blohs and Moissl-Eichinger.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogenetic tree based on the retrieved RSVs from the universal approach with the primer pairs 515F-806uR and 519F-785R, archaeal approach with primer pair 519F-806R and from the PCR based on the primer pair combination 344F-1041R/519F-806R as indicated in colors as an outer circle [legend “Primer combinations (PCR)”]. The inner circle represents the body site from where the RSVs were identified (see legend). Reference sequences from the SILVA database are shown without label/circle. The branches of the tree were colored according to the phyla: blue, Woesearchaeota; green, Euryarchaeota; and orange, Thaumarchaeota.