TABLE 2.
displays all primer pair combinations used for the first and the second PCR of the nested approach and the “universal” PCR.
| PCR # | Primer combination 1st PCR | Primer combination 2nd PCR |
| PCR21 | 349F-915R | Illu 349F-Illu519R |
| PCR22 | 349F-915R | Illu 519F-Illu785R |
| PCR23 | 349F-915R | Illu 519F-Illu806R |
| PCR31 | 344F-1041R | Illu 349F-Illu519R |
| PCR33 | 344F-1041R | Illu 519F-Illu785R |
| PCR34 | 344F-1041R | Illu 519F-Illu806R |
| PCR41 | 349F-1041R | Illu 349F-Illu519R |
| PCR42 | 349F-1041R | Illu 519F-Illu785R |
| PCR43 | 349F-1041R | Illu 519F-Illu806R |
| PCR61 | 349F-806R | Illu 349F-Illu519R |
| PCR62 | 349F-806R | Illu 519F-Illu785R |
| PCR63 | 349F-806R | Illu 519F-Illu806R |
| PCR71 | 519F-1041R | Illu 519F-Illu785R |
| PCR72 | 519F-1041R | Illu 519F-Illu806R |
| PCR81 | 519F-806R | Illu 519F-Illu785R |
| PCR82 | 519F-806R | Illu 519F-Illu806R |
| PCR91 | 344F-519R | Illu 349F-Illu519R |
| PCRQ1 | 344F-915R (QIAGEN) | Illu 349F-Illu519R |
| PCRQ3 | 344F-915R (QIAGEN) | Illu 519F-Illu785R |
| PCRQ4 | 344F-915R (QIAGEN) | Illu 519F-Illu806R |
| PCRM1 | 344F-915R (NEB Monarch) | Illu 349F-Illu519R |
| PCRM3 | 344F-915R (NEB Monarch) | Illu 519F-Illu785R |
| PCRM4 | 344F-915R (NEB Monarch) | Illu 519F-Illu806R |
| PCRA1 | 344F-915R (Analytik Jena) | Illu 349F-Illu519R |
| PCRA3 | 344F-915R (Analytik Jena) | Illu 519F-Illu785R |
| PCRA4 | 344F-915R (Analytik Jena) | Illu 519F-Illu806R |
| PCRQ5 | 344F-806R (QIAGEN) | Illu 349F-Illu519R |
| PCRQ6 | 344F-806R (QIAGEN) | Illu 519F-Illu785R |
| PCRQ7 | 344F-806R (QIAGEN) | Illu 519F-Illu806R |
| PCRM5 | 344F-806R (NEB Monarch) | Illu 349F-Illu519R |
| PCRM6 | 344F-806R (NEB Monarch) | Illu 519F-Illu785R |
| PCRM7 | 344F-806R (NEB Monarch) | Illu 519F-Illu806R |
| PCR8-Uni | n.a. | Illu 515F-Illu806uR |
| PCR9-Uni | n.a. | Illu 515FB-Illu806RB |
| PCR10 | n.a. | Illu 519F-Illu806R |
| PCR11-Uni | n.a. | Illu 519F-Illu785R |
If not indicated otherwise (in brackets), the first PCR was followed by a purification of the PCR product by the MinElute PCR Purification kit (QIAGEN) kit. n.a., not applicable.