TABLE 4.
In silico analysis of the coverage of chosen primer pairs for specific archaeal taxa of interest.
|
Euryarchaeota |
Thaumarchaeota |
Nanoarchaeota |
||||||
| Primer pair | Name | Total | Methanobrevibacter | Methanosphaera | Methanomassiliicoccus | Total | Nitrososphaera | Woesearchaeota |
| 1 | 344F | 89.80% | 94.90% | 81.00% | 100% | 20.40% | 87.10% | 55.80% |
| 915R | ||||||||
| 2 | 349F | 89.70% | 95.00% | 83.00% | 100% | 91.2% | 89.30% | 70.30% |
| 915R | ||||||||
| 3 | 344F | 89.90% | 94.30% | 78.20% | 100% | 20.60% | 89.00% | 56.60% |
| 1041R | ||||||||
| 4 | 349F | 90.20% | 94.40% | 78.60% | 100% | 95.80% | 92.30% | 73.40% |
| 1041R | ||||||||
| 5 | 519F | 94.60% | 97.40% | 84.60% | 92.90% | 96.50% | 90.60% | 82.40% |
| 1041R | ||||||||
| 6 | 344F | 91.50% | 95.20% | 82.20% | 100% | 23.20% | 88% | 55.00% |
| 806R | ||||||||
| 7 | 349F | 91.40% | 95.30% | 84.20% | 100% | 96.10% | 90.10% | 72.60% |
| 806R | ||||||||
| 8 | 519F | 96.30% | 98.60% | 90% | 95% | 96.50% | 89.50% | 82.90% |
| 806R | ||||||||
| 9 | 349F | 91.90% | 95.60% | 90.30% | 95% | 97.50% | 94.40% | 83.10% |
| 519R | ||||||||
| 10 | 519F | 96.20% | 98.40% | 89.60% | 95% | 96.00% | 86.90% | 87.30% |
| 785R | ||||||||
| 11 | 515F | 95.90% | 98.30% | 89.60% | 95% | 94.60% | 86.90% | 89.10% |
| 806R | ||||||||
| 12 | 515FB | 95.90% | 98.30% | 89.60% | 95% | 96.40% | 89% | 89.10% |
| 806RB | ||||||||
One mismatch was allowed per primer (last base at the 3′ end was excluded for mismatch). For primer full names and sequences, please refer to Table 1.