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. 2019 Dec 5;10:2803. doi: 10.3389/fmicb.2019.02803

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Copyright © 2019 Nabb, Song, Kluthe, Daubert, Luedtke and Nuxoll.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Matrix coating does not inhibit vancomycin binding. (A) Vancomycin BODIPY FL conjugate was added to planktonic cells in either polymicrobial (red) or monomicrobial (blue) cultures. Vancomycin was able to bind S. aureus similarly in both conditions. Unstained cells were included as a control (gray). (B) Fluorescence from delafloxacin either in polymicrobial (red) or monomicrobial (blue) cultures was unable to be differentiated from unstained cells (gray) due to the weak signal produced. (C) To ensure matrix coating occurred, ConA was added to polymicrobial (red) cultures and compared to unstained polymicrobial cultures (gray). Data is representative of three independent replicates.