FIGURE 9.

Fumarase C Expression. GFP expression of PfumC:gfp (A) and Pspa:gfp (B) was measured over time using a Biotek microplate reader. Overnight cultures of S. aureus (1:100) and C. albicans (1:50) were diluted in MHB in a microtiter plate. C. albicans decreased expression of the TCA cycle gene, fumarase (red) compared to expression observed when S. aureus was grown alone (blue). This effect was specific to fumarase and not the result of a generalized reduction in S. aureus transcription as indicated by the Pspa:gfp control. C. albicans did not affect fluorescence outside of gene expression (black). Experiments were performed in biological triplicate; error bars represent standard deviation.