Fig. 1.

Amplicon sequencing of CACNA1C. a UCSC genome browser screenshot of CACNA1C isoforms annotated in GENCODE V27. All transcripts in “Basic” annotated set shown. Black arrow shows direction of transcription. TSS transcription start site. Position of forward and reverse long-range PCR primers in first exon and universal portion of final exon shown. Length vs Quality of all 2D pass reads from b Run 1 c Run 2. Most reads are the full-length CACNA1C CDS. The presence of the 3.5 kb positive control CDS spike-in can be seen in Run1. Visualisation limited to reads between 2 and 8 kb, encompassing >98% of pass reads in each run