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. 2019 Dec 5;10:1478. doi: 10.3389/fphar.2019.01478

Figure 6.

Figure 6

Potential anti-inflammatory mechanism of FGF-1 in insulin resistance. (A) Schematic diagram of TAK1-TAB1-mediated JNK signaling pathway being essential for regulating inflammation. (B–C) The interaction between TAK1 and TAB1 was detected by co-immunoprecipitation in HEK293T cells. (D) Total cell lysates from RAW 264.7 cells treated with 10 ng/ml FGF-1 or control for 1 h prior to 50 ng/ml TNFα for 15 min were immunoprecipitated with an antibody to TAK1, followed by immunoblotting. (E–F) Total liver lysates from mice treated with FGF-1 or control were immunoprecipitated with an antibody to TAK1, followed by immunoblotting. TCL, total cell lysate. Uncropped blots can be found in Supplementary Figure 5 .