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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Diagn Microbiol Infect Dis. 2019 Sep 9;96(1):114894. doi: 10.1016/j.diagmicrobio.2019.114894

Table 1.

Primer and probe sequences for OROV assays.

Name Sequence (5’ → 3’) Conc.a Locationb
OROV rRT-PCR
  OROV Forward-S GACAAGTSCTCAATGCTGGTGT 200nM 92–113
  OROV Forward-K GACAAGTGCTCAATGCTKGTGT 200nM
  OROV Reverse CGTTGTCCGGSACTGGATT 200nM 247–265
  OROV Probe-Yc TGGTTGACCTYACTTTTGGTGGGGT 200nM 179–203
  OROV Probe-Rc TGGTTGACCTTACTTTTRGTGGGGT 200nM
Comparator rRT-PCR
  Forward Primer CATTTGAAGCTAGATACGGACAA 1,000nM 74–96
  Reverse Primerd GGCACTGGATTCGACTGGA 1,000nM 239–257
  Probec CAATGCTGGTGTTGTTAGAGTCTTCTTCCT 600nM 102–230
a

Concentration of each oligonucleotide in the final reaction mixture

b

Genomic locations for viral primers and probes are provided based on the following reference sequences: Oropouche virus isolate H759483 (GenBank: HQ830492.1).

c

5’ fluor and 3’ quencher pairs were FAM and BHQ-1

d

Reverse primer was redesigned from the assay by Weidmann, et al., 2003