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Antibody and staining information. Abbreviations: HIER - heat-induced epitope retrieval.
| Antibody name | Clone | Vendor | Catalog No. | Concentration (μg/ml) | TMA tested | Antigen Retrieval |
|---|---|---|---|---|---|---|
| Cytokeratin 5/6 | D5/16 B4 | Dako | M7237 | 0.9 | TMA #2 | HIER, pH 9 |
| EGFR | 31G7 | Invitrogen | 28–0005 | 0.6 | TMA #2 | Proteinase K |
| ER | SP1 | Cell Marque | 249R-15 | 0.5 | TMA #1 | HIER, pH 9 |
| HER2 | CB11 | BioGenex | MU134-UC | 0.2 | TMA #1 | HIER, pH 9 |
| Ki67 | MIB-1 | Dako | GA626 | Ready to use | TMA #2 | HIER, pH 6 |
| PR | Y85 | Cell Marque | 323R-15 | 0.4 | TMA #1 | HIER, pH 9 |
