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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Appl Immunohistochem Mol Morphol. 2020 Apr;28(4):267–273. doi: 10.1097/PAI.0000000000000756

Table 2.

Significant differences between stored slides and the fresh cut slide. Only storage conditions and durations with a significant difference are listed. For ER, PR, and HER2, there were no significant differences between the number of positive cases for fresh cut and stored slides, regardless of the storage condition or duration of storage. Kappa, K, measures agreement between the stored and fresh sections.

Storage condition Duration of storage P K (95% CI)
Ki67 paraffin dipped 1 month 0.02 0.57 (0.33–0.81)
CK5/6 4C 1 week 0.03 0.78 (0.61–0.96)
−20C 1 week 0.03 0.65 (0.44–0.86)
−80C 1 week 0.03 0.67 (0.47–0.87)
EGFR paraffin dipped 1 week 0.005 0.51 (0.31–0.71)
paraffin dipped 1 month <0.0001 0.27 (0.09–0.46)
paraffin dipped 6 months 0.001 0.39 (0.19–0.59)
−20C 6 months 0.02 0.61 (0.43–0.79)