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. 2019 Dec 11;9:18845. doi: 10.1038/s41598-019-55449-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Exogenous TCTP relieves the decline of oocyte quality. Oocytes were treated with mCherry or TCTP-mCherry for 4 hours. After washing the proteins, oocytes were in vitro cultured in fresh media for 24 hours. (A–C) Spindle microtubules and chromosomes were immunostained with anti-tubulin antibody and DAPI, respectively. Representative images from three independent experiments are shown in (A). Bar, 20 μm. (B,C) Percentage of lagging chromosomes and maximal spindle length are shown. Data are expressed as means ± SEMs from three independent experiments. (D,E) Oxidative stress levels were determined by measuring fluorescence intensity following incubation with DCF-DA for 30 min. Representative images from three independent experiments are shown, along with quantification of ROS levels. Bar, 80 μm. (F,G) Mitochondrial distribution was determined by immunostaining with CytoPainter MitoRed. Representative images from three independent experiments are shown. Bar, 40 μm. The incidence of mitochondrial aggregation is shown. Data are expressed as means ± SEMs from three independent experiments. *p < 0.05, **p < 0.001, ***p < 0.0001 (Student’s t-test).