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. 2019 Dec 11;9:18907. doi: 10.1038/s41598-019-55329-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Noise-induced decreases in τ_bound in IHCs and IPCs result from an increase in NADH in these cells relative to OPCs. (A) NAD(P)H fluorescence intensity of each cell type under control and noise-treated conditions, normalised in each image to the mean OPC level. These cells were the most metabolically stable population following noise exposure, based on the smallest changes in NAD(P)H fluorescence decay parameters. (B,C) Corresponding NADH and NADPH concentrations, again relative to OPCs, calculated using established procedures³⁴ from the fluorescence intensity and decay parameters.