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. 2019 Dec 11;9:18907. doi: 10.1038/s41598-019-55329-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Development and ageing cause distinct patterns of τ_bound variation in HCs and PCs. (A–D) NAD(P)H fluorescence intensity images of each cell type at varying postnatal ages (scale bar 10 µm). (E–H) Mean enzyme bound NAD(P)H fluorescence lifetimes τ_bound (*P < 0.05). (I–L) FLIM images colour coded for the mean τ_bound value in each cellular region of interest. OHC data is truncated at 1Y due to early-onset hearing loss in C57BL/6 mice.