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. 2019 Dec 11;10(12):947. doi: 10.1038/s41419-019-2148-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a The hUC-MSCs were cultured in normal growth medium (Undiff) or osteogenic differentiation medium (Diff) for 21 days, and analyzed by fluorescence laser confocal for cytoskeleton (F-actin), cell membrane (Dil) and nucleus (DAPI). Scale bar, 20 μm. b–c The diff and undiff hUC-MSCs cells were analyzed by Alizarin red S (ARS) and alkaline phosphatase (ALP) staining. Scale bar, 200 μm. d An operating flow diagram for lncRNA microarray and a heatmap for differentially expressed lncRNAs of osteogenic differentiated hUC-MSCs compared to undifferentiated hUC-MSCs. ENST00000520431 (ODIR1) was significantly decreased after osteogenic differentiation. e The hUC-MSCs were incubated for 7, 14, and 21 days, respectively, then the RNA levels of ODIR1, CD44, OSX, RUNX2, and OPN were analyzed by RT-qPCR, and the proteins levels of CD44, OSX, RUNX2, OPN were analyzed by western blots. GAPDH was used as internal controls.