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. 2019 Dec 11;10(12):947. doi: 10.1038/s41419-019-2148-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b The hUC-MSCs were cultured in normal growth medium (Undiff) or in osteogenic differentiation medium (Diff) for 10 days. The antibodies H2AK119ub, H2BK120ub, H3K4me3, and IgG were incubated with cell lysates, respectively, and the enrichment of those three histone markers on OSX or RUNX2 promoter was analyzed by ChIP-qPCR. c, d The hUC-MSCs were transfected with NC and ODIR1 siRNAs. The antibodies H2AK119ub, H2BK120ub, H3K4me3, and IgG were incubated with cell lysates, respectively, and the enrichment of those three histone markers on OSX or RUNX2 was analyzed by ChIP-qPCR. e The pMIR-OSX-luc (containing OSX promoter region) or pMIR-RUNX2-luc (containing RUNX2 promoter region) plasmids were co-transfected with pCDH-ODIR1 or pCDH plasmids into 293 cells, respectively, and the cell lysates were analyzed by luciferase assay. f The pMIR-OSX-luc plasmid was co-transfected with NC and ODIR1 siRNAs into 293 cells, respectively, and the cell lysates were analyzed by luciferase assay. g The pMIR-OSX-luc plasmids were co-transfected with different amounts (0.1, 0.2, 0.5, 1.0 μg) of pCDH-ODIR1 plasmids into 293 cells, respectively, and analyzed by luciferase assay.