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. 2019 Dec 11;9:18803. doi: 10.1038/s41598-019-55088-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of serum titers of antibodies against CF4-C12L and C3-C12L in mice immunized with CF4-C12L. Mice were immunized with CF4-C12L, and serum was prepared 3 days (D3) or 7 days (D7) after the final immunization. BG, serum from non-treated mice. Titers of serum IgM (left panel) and IgG (right panel) against CF4-C12L (closed squares with solid line) and C3-C12L (open circles with dotted line) were determined by ELISA. Error bars: mean ± S.D. (n = 6). *P < 0.05 anti-CF4-C12L vs. anti-CF3-C12L.