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. 2019 Dec 11;9:18803. doi: 10.1038/s41598-019-55088-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Serum antibody titers in CF4-C182L– and CF3-C182L–immunized mice. Mice were immunized with CF4-C182L or CF3-C182L, and serum was prepared 3 (open bars) or 7 (gray bars) days after the final immunization. Closed bars, serum from non-treated mice. Titers of serum IgM (A) and IgG (B) against each immunogen were determined by ELISA. (C) Titers of serum IgM against thyroglobulin. Titers of serum antibodies in CF4-C12L–immunized mice are shown for reference. Error bars: mean ± S.D. (n = 6–7). *P < 0.05, **P < 0.01, **P < 0.001 BG vs. D3 or D7.