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. 2019 Dec 11;9:18829. doi: 10.1038/s41598-019-55100-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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miR-146a reduces the production of antiviral cytokines and suppresses the function of T cells. CD8+ T cells from healthy individuals were transfected with 50 nmol/ml miR-146a mimic or miR-146a inhibitor, a randomized oligonucleotide served as a mock, and cultured in 1 mg/ml anti-CD3 for 48 h. (a) The relative mRNA levels of IFN-γ, IL-2, and TNF-α after transfected with miR-146a mimic and miR-146a inhibitor were assessed for real-time PCR using GAPDH as endogenous control. The levels of IFN-γ, IL-2, and TNF-α in the supernatant were detected by ELISA. (b) Quantitative PCR for GZMB, perforin and CD107a mRNA relative levels after transfected with miR-146a mimic or miR-146a inhibitor. Data shown as mean ± SEM. *p < 0.05, **p < 0.01.