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. 2019 Dec 11;10:5647. doi: 10.1038/s41467-019-13699-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Representative images from a CX3CR1GFP × CCR2RFP mouse before and after DSS treatment (administered on Day 0). Using a combination of tattoo and vascular mapping we track innate dendritic cells and inactivated monocytes (green), activated monocytes (blue), and vasculature (red) in the same position for 5 days. b Representative images from a Lgr5-eGFP mouse before and after irradiation (treated on Day 0), demonstrating reduction in colonic crypts (green) after irradiation. Location is tracked by tattoo and vasculature branch points (white dashed lines) without vasculature dye. c Representative images from a Lgr5-eGFP mouse before and after irradiation with individual Lgr5-positive cells (green) and crypts labeled (white dashed lines). Location is tracked by tattoo and vasculature (red). d, e Quantification of Lgr5-positive d) crypt area (μm²) and e) cells per crypt after irradiation (administered on Day 0). Circles represent individual mice by color, and bars indicate μ ± s.e.m. Star indicates statistically significant difference from control by Dunnett’s test for multiple comparisons after repeated-measures ANOVA (n ≥ 4, p < 0.001). All scale bars are 100 μm.