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. 2019 Dec 11;9:18806. doi: 10.1038/s41598-019-54933-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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A typical in vivo L-COSY acquired from the PCG using a 3 T (Prisma) equipped with a 64 channel head and neck coil is shown expanded (F2: 0–5 ppm; F1: 0–5 ppm) to inspect regions of interest. The acquisition parameters were voxel size 30 × 30 × 30 mm³, increment size 0.8 ms, increments 96, 8 averages per increment, TR 1.5 sec, total experimental time 19:12 min, acquired vector: 1024 points, acquisition time: 512 ms, spectral width in F2: 2000 Hz, spectral width in F1: 1250 Hz. Abbreviations: N-acetyl aspartate (NAA), choline (Cho); creatine (Cr); glutamate and glutamine together (Glx); aspartate (Asp); myo-inositol (m-Ino); histidine (His); lactate (Lac); γ-aminobutyric acid (GABA); macromolecule (MM); glutathione (GSH); threonine (Thr); phosphoryl ethanolamine (PE). The region highlighted by the green box contains the crosspeaks assigned to the fucosylated glycans and is expanded in Fig. 3 as both a contour plot and 3D plot of the region where the fucosylated glycans resonate.