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. 2019 Dec 11;9:18830. doi: 10.1038/s41598-019-55027-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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E2F2 overexpression induces adult mouse cardiomyocyte (CM) cell death. (a) E2F2 was expressed by adenovirus vector at various multiplicity of infection (MOI). Cell viability is visualized by the transgenic tdTomato expression (red) at day 7 post-transduction and quantified on the right. (b) Longitudinal imaging of CMs revealed cell death in the E2F2-expressing group. Error bars represent standard deviation from duplicates in a single experiment. Scale bars, 100 μm.