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. 2019 Dec 11;9:18852. doi: 10.1038/s41598-019-55189-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of selective and nonspecific NOX inhibitors on platelet aggregation. Washed platelets (3.6 × 10⁸ cells/ml) were pre-incubated with DMSO (solvent control), (A) GKT136901 (GKT1, 20 μM), (B) GKT137831 (GKT2, 20 μM), (C) GSK2795039 (GSK, 20 μM), or (D,E) combination of ML171 (ML, 0.5 μM), GSK (20 μM), and GKT1 or GKT2 (20 μM) following stimulation with collagen (1 μg/ml), convulxin (10 ng/ml), thrombin (0.02 U/ml) and U46619 (1 μM) to trigger platelet aggregation. Profiles (A–E) are representative examples of three similar experiments.