Table 1.

Rates of abnormal embryos.

Experiment	Group	Total embryos	Abnormal embryos	Abnormal rate % (mean ± SE)
1	No injection	3146	55	1.75 ± 0.08 a
	pSuperpuro	2742	103	3.75 ± 0.11 a
	shRNAmis	1326	50	3.77 ± 0.35 a
	shRNAran	1146	20	1.75 ± 0.02 a
	shRNA1	3566	362	10.15 ± 0.30 b
	shRNA2	7153	1264	17.67 ± 1.52 c
2	Control	2888	55	1.90 ± 0.09 a
	shRNA2	3098	505	16.30 ± 0.58 b
	shRNA2 + mRNA	2205	104	4.72 ± 0.31 c

Note: Significant differences between groups are shown by different letters following the data (P < 0.05). The shRNAs – shRNA1, shRNA2 – target nucleotide 77–99 and 217–237 of Drfundc1 ORF respectively. shRNAmis, mismatched shRNA; shRNAran, random shRNA without any target gene.