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. 2019 Dec 11;9:18869. doi: 10.1038/s41598-019-55269-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of C3H10T1/2/IFN-γ cells or C3H10T1/2/HSVtk/IFN-γ cells on the proliferation of colon26/luc cells. (A) Viability of colon26/luc cells on incubation with the conditioned medium of C3H10T1/2/IFN-γ or C3H10T1/2/HSVtk/IFN-γ cells. The conditioned media of C3H10T1/2/IFN-γ or C3H10T1/2/HSVtk/IFN-γ cells were collected after 48 h incubation. The medium of colon26/luc cells was replaced with the conditioned media and the cells were cultured for 48 h. The viability of colon26/luc cells was calculated using CCK-8. Results are expressed as the mean ± SD of four samples. A representative of four independent experiments with similar results is shown. *p < 0.05; statistically significant differences observed in comparison with no treatment group. (B) Viability of colon26/luc cells after co-culture of C3H10T1/2/IFN-γ or C3H10T1/2/HSVtk/IFN-γ cells with colon26/luc cells. C3H10T1/2/IFN-γ or C3H10T1/2/HSVtk/IFN-γ cells and colon26/luc cells were co-cultured for 48 h. The viability of colon26/luc cells was calculated by detecting the luminescence of colon26/luc cells. Results are expressed as the mean ± SD of four samples. A representative of ten independent experiments with similar results is shown. *p < 0.05; statistically significant differences observed in comparison with no treatment group.