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. 2019 Dec 11;9:18779. doi: 10.1038/s41598-019-55339-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Changes in bodyweight (expressed in g) over the age of female and male (A) Cushing’s (het) mice and (B) Afmid (hom) mice compared with their respective WT littermates. Highlighted in green (het); highlighted in red (hom); highlighted in blue (WT) (C) Blood glucose concentrations expressed in mmol L⁻¹ of Cushing’s mice (het) compared with WT littermates, age- and sex-matched groups (D) plasma insulin concentrations expressed in ng mL⁻¹ of Cushing’s mice (het) compared with WT littermates, age- and sex-matched groups. P values were calculated between age- and sex-matched groups using student’s t-test and one-way repeated measures ANOVA with Bonferroni post-hoc test (^◆p < 0.05, ^◆◆p < 0.001); Mann Whitney’s test (^#p < 0.05). Outliers are represented with a circle (◦) and extreme points are indicated with an asterisk (*). Error bars represent SEM (standard error of mean).