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. 2019 Dec 11;9:18779. doi: 10.1038/s41598-019-55339-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PLS-DA score plots discriminating between WT littermates and Cushing’s (het) mice at (A) 12 weeks of age including Feature Selection (FS), data acquired using SIFT-MS; (B) 16 weeks of age, data acquired by TD-GC-MS. Highlighted in blue is shown the WT littermates and Cushing’s (het) diabetic animals are assigned in red. Number of independent observations and number of variables is given in Supplementary Information (Tables S2 and S3).