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. Author manuscript; available in PMC: 2019 Dec 12.
Published in final edited form as: Anal Biochem. 2001 Jul 15;294(2):118–125. doi: 10.1006/abio.2001.5155

FIG. 6.

FIG. 6.

Excitation wavelength dependence of the apparent pKa of LysoSensor yellow/blue DND-160. When excitation wavelength was adjusted from 380 and 365 to 350 nm, the apparent pKa dropped from4.5 and 3.8 to 3.1. The modulation frequency was fixed at 194.3 MHz.