Table 1.
Primer | Gene Position | Sequence 5′-3′ | Annealing Temperature (oC) |
---|---|---|---|
1F1 | 1–21 | atggctgcktgagacacacgt | 62.8 |
1R525 | 525–548 | cgtgtacagcatagacgtcttggt | |
1F409 | 409–432 | catytctggaaagatcggggactt | 62.5 |
1R819 | 819–842 | taagtagcgtgcggctttccgggt | |
1F804 | 804–825 | tagggtcaacgctttacccgga | 63.9 |
1R1375 | 1375–1398 | tggattgactgggtatcaggcctc | |
1F1311 | 1311–1334 | aaagaacactaacctgctgctgtc | 63.3 |
1R1867 | 1867–1890 | aggactcggccatcgtacgcttcg | |
1F1800 | 1800–1822 | tgatccacgctttagcggagcaa | 63.6 |
1R2375 | 2375–2398 | caagagcagcgaatctaccgtacg | |
1F2320 | 2320–2340 | ctgccaagaaatcagcaccga | 61.5 |
1R2874 | 2874–2896 | tgatgcatagagtgggttttcgt | |
1F2801 | 2801–2824 | cacgaggtcatgacagcagccgca | 62.7 |
1R3365 | 3365–3388 | ttctagaatggacgctgcctcagg | |
1F3303 | 3303–3324 | ctgtgtattacgcggataacca | 61.2 |
1R3822 | 3822–3843 | gagccacccggtttgagcagtc | |
1F3808 | 3808–3829 | gggtgactcactgagactgctc | 62.5 |
1R4328 | 4328–4351 | gacttctcgataggcagccgccaa | |
1F4320 | 4320–4341 | accgggaattggcggctgccta | 64.7 |
1R4877 | 4877–4900 | tcgggtgacgcgttcaggagtcat | |
1F4800 | 4800–4822 | tcaggcagaaatgcccggtggat | 64.6 |
1R5368 | 5368–5389 | tacggccggacacagctctgct | |
1F5218 | 5218–5241 | cataatcggaaaccttgcggccgt | 64.4 |
1R5832 | 5832–5855 | acctgcttctattggccatggacg | |
1F5818 | 5818–5838 | actccaggagagtgcgtccat | 61.9 |
1R6317 | 6317–6340 | cacgttgaatactgctgagtccaa | |
1F6303 | 6303–6326 | gggaattacccactttggactcag | 61.3 |
1R6845 | 6845–6865 | cagggagtgatccactcccaa | |
1F6700 | 6700–6720 | atttgacatgtctgccgagga | 61.7 |
1R7222 | 7222–7243 | tagcgggtccgccactctgcaa | |
1F7240 | 7204–7226 | tactgtgacaggaacagcttgca | 62.7 |
1R7719 | 7719–7714 | gttgaggtaccgcgcattgtc | |
1F7716 | 7716–7737 | actgacaatgcgcgcggtacct | 64.5 |
1R8275 | 8275–8296 | tcaccaccgagagggctgtacg | |
1F8201 | 8201–8221 | acagcggtagaccgatcttcg | 63.7 |
1R8741 | 8741–8764 | gcatgtgattgtccatgtaacgca | |
1F8721 | 8721–8741 | tagccatgattggaccaagct | 62.3 |
1R9213 | 9213–9236 | tgattggtgaccgcggcatggcat | |
1F9204 | 9204–9227 | ggttgatcaatgccatgccgcggt | 64.3 |
1R9741 | 9741–9764 | acggtagctcctggtgttcagttcg | |
1F9702 | 9702–9722 | gatgtgcatgtgtgcacgacg | 63.5 |
1R10236 | 10236–10259 | cacatgaatgggtagacgccggtg | |
1F10102 | 10102–10122 | actttggagccaacgctatcg | 62.9 |
1R10677 | 10677–10700 | gcctgagagtacggcacgtgcacc | |
1F10600 | 10600–10620 | atccaaagtcgcacgcctgag | 64.3 |
1R11179 | 11179–11199 | aatgtcttggaccccgagggt | |
1F11113 | 11113–11136 | gtacactgtgcagccgagtgccat | 60.0 |
1R11811 | 11811–11834 | agccactttgaacatctcctacgt | |
1F11200 | 11200–11223 | tccgctacggcgatgtcatgggtg | 59.3 |
1R11811 | 11811–11834 | agccactttgaacatctcctacgt | |
1F11313 | 11313–11335 | acttgacaactaggtacgaaggt | 57.3 |
1R11811 | 11811–11834 | agccactttgaacatctcctacgt |