Skip to main content
. 2019 Dec 7;5(12):e02682. doi: 10.1016/j.heliyon.2019.e02682

Table 1.

Set of primers used in RT-PCR.

Primer Gene Position Sequence 5′-3′ Annealing Temperature (oC)
1F1 1–21 atggctgcktgagacacacgt 62.8
1R525 525–548 cgtgtacagcatagacgtcttggt
1F409 409–432 catytctggaaagatcggggactt 62.5
1R819 819–842 taagtagcgtgcggctttccgggt
1F804 804–825 tagggtcaacgctttacccgga 63.9
1R1375 1375–1398 tggattgactgggtatcaggcctc
1F1311 1311–1334 aaagaacactaacctgctgctgtc 63.3
1R1867 1867–1890 aggactcggccatcgtacgcttcg
1F1800 1800–1822 tgatccacgctttagcggagcaa 63.6
1R2375 2375–2398 caagagcagcgaatctaccgtacg
1F2320 2320–2340 ctgccaagaaatcagcaccga 61.5
1R2874 2874–2896 tgatgcatagagtgggttttcgt
1F2801 2801–2824 cacgaggtcatgacagcagccgca 62.7
1R3365 3365–3388 ttctagaatggacgctgcctcagg
1F3303 3303–3324 ctgtgtattacgcggataacca 61.2
1R3822 3822–3843 gagccacccggtttgagcagtc
1F3808 3808–3829 gggtgactcactgagactgctc 62.5
1R4328 4328–4351 gacttctcgataggcagccgccaa
1F4320 4320–4341 accgggaattggcggctgccta 64.7
1R4877 4877–4900 tcgggtgacgcgttcaggagtcat
1F4800 4800–4822 tcaggcagaaatgcccggtggat 64.6
1R5368 5368–5389 tacggccggacacagctctgct
1F5218 5218–5241 cataatcggaaaccttgcggccgt 64.4
1R5832 5832–5855 acctgcttctattggccatggacg
1F5818 5818–5838 actccaggagagtgcgtccat 61.9
1R6317 6317–6340 cacgttgaatactgctgagtccaa
1F6303 6303–6326 gggaattacccactttggactcag 61.3
1R6845 6845–6865 cagggagtgatccactcccaa
1F6700 6700–6720 atttgacatgtctgccgagga 61.7
1R7222 7222–7243 tagcgggtccgccactctgcaa
1F7240 7204–7226 tactgtgacaggaacagcttgca 62.7
1R7719 7719–7714 gttgaggtaccgcgcattgtc
1F7716 7716–7737 actgacaatgcgcgcggtacct 64.5
1R8275 8275–8296 tcaccaccgagagggctgtacg
1F8201 8201–8221 acagcggtagaccgatcttcg 63.7
1R8741 8741–8764 gcatgtgattgtccatgtaacgca
1F8721 8721–8741 tagccatgattggaccaagct 62.3
1R9213 9213–9236 tgattggtgaccgcggcatggcat
1F9204 9204–9227 ggttgatcaatgccatgccgcggt 64.3
1R9741 9741–9764 acggtagctcctggtgttcagttcg
1F9702 9702–9722 gatgtgcatgtgtgcacgacg 63.5
1R10236 10236–10259 cacatgaatgggtagacgccggtg
1F10102 10102–10122 actttggagccaacgctatcg 62.9
1R10677 10677–10700 gcctgagagtacggcacgtgcacc
1F10600 10600–10620 atccaaagtcgcacgcctgag 64.3
1R11179 11179–11199 aatgtcttggaccccgagggt
1F11113 11113–11136 gtacactgtgcagccgagtgccat 60.0
1R11811 11811–11834 agccactttgaacatctcctacgt
1F11200 11200–11223 tccgctacggcgatgtcatgggtg 59.3
1R11811 11811–11834 agccactttgaacatctcctacgt
1F11313 11313–11335 acttgacaactaggtacgaaggt 57.3
1R11811 11811–11834 agccactttgaacatctcctacgt