Fig. 9.

Paxillin-EGFP signal intensity in adhesions decreases upon the addition of a ROCK inhibitor. TIRF images of a representative CHO-K1 cell expressing: (A, B) paxillin-EGFP acquired before (A) and after (B) treatment with Y-27632, (C, D) paxillin-EGFP in cells also transfected with βPIX-mCherry (not shown) acquired before (C) and after (D) treatment with Y-27632, and (E, F) paxillin-EGFP acquired before (E) and after (F) the addition of ddH₂O (control). Scale bars are 10 µm. (G) The average intensity of adhesions within cells before and after treatment with Y-27632 or ddH₂O (control). Nine cells from at least three independent experiments were averaged in the Y-27632 treatment and ten cells, from four different experiments, were averaged in the control. There were a total of 2104 adhesions before Y-27632 treatment and 1784 adhesions after for the paxillin-EGFP cells, 4772 adhesions before treatment and 4510 adhesions after for the paxillin-EGFP and βPIX-mCherry cells, and 5873 adhesions before treatment and 6315 adhesions after for the control cells. Statistical significance was calculated using a t-test on the percentage change (relative change of the after condition to the before condition) and is indicated by stars, with four stars (****) indicating P < 0.0001 and three stars (***) corresponding to P < 0.001. Error bars represent standard errors of the means.