Figure 1.

Existence of circAMOTL1 in Cervical Cancer Cells

(A) Data from the UCSC website revealed that AMOTL1 was the host gene for circAMOTL1. (B) The qPCR analysis was applied to validate the expression of circAMOTL1in cervical cancer cell lines. (C) The “head-to-tail” splicing sites of circAMOTL1 detected by Sanger sequencing. (D) The expression level of circAMOTL1 and linear AMOTL1 in CaSki cells treated with Actinomycin D in different time points. (E) The expression level of circAMOTL11 and AMOTL1 mRNA in CaSki cells treated with or without Rnase R was measured by qRT-PCR. (F) The localization of circAMOTL1 in CaSki cell was evaluated by FISH assay. Scale bar, 200 μm. **p < 0.01. n.s.: no significance.