Figure 1.

ODF1 immunodetection by western blot, in proteins obtained from testis (T), kidney total fraction (K), liver (L), kidney cortex-cytoskeleton (KC–C) kidney medulla-cytoskeleton (KM-C), kidney cortex- non cytoskeleton (KC-NC) kidney medulla- non cytoskeleton (KM-NC). Different antibodies were used: in panel a: an antibody raised against a peptide mapped near the N-terminus and in panel ban antibody raised against a peptide mapping within an internal region, of ODF1 of human origin. In panel c an antibody against α-tubulin was used. On the left the approximate molecular weight was indicated. The results are representative from three separated experiments. In panel d: Odf1-mRNA expression detected by RT-PCR. In panel e: β actin-mRNA expression detected by RT-PCR. From left to right: PBM (pair base marker), T, K and L. The results are representative from two separated experiments. In panel f: optical density ratio of ODF1 relative to β-ACTIN and normalized to testis expression.