Figure 1.

linc00174 Was Highly Expressed in GECs, and Knock Down of linc00174 Increased BTB Permeability and Induced the Expression of Tight Junction-Related Proteins in GECs

(A and B) lncRNA (A) and miRNA (B) microarray data in AECs and GECs; quantitative real-time PCR were performed to validate the selected molecules. (C) Relative expression levels of linc00174 in AECs and GECs were detected by quantitative real-time PCR. Data represent mean ± SD (n = 5, each). **p < 0.01 versus AECs group. (D) TEER values were evaluated after knock down of linc00174 in GECs. (E) HRP flux assays were performed to detect permeability after knock down of linc00174 in GECs. Data represent mean ± SD (n = 5, each). *p < 0.05 versus linc00174(−) group. (F) Western blot analysis for the protein expression of tight junction-related proteins ZO-1, occludin, and claudin-5 in GECs. Data represent mean ± SD (n = 3, each). *p < 0.05 versus linc00174(−) group. (G) Immunofluorescence localization of ZO-1, occludin, and claudin-5 in GECs. Nuclei are labeled with DAPI. Images are representative of three independent experiments. Scale bar, 50 μm.