Figure 5.

miR-138-5p/miR-150-5 Targeted FOSL2 3′ UTR

(A) Relative expression of FOSL2 mRNA was detected by quantitative real-time PCR in GECs after knock down of linc00174. (B) Relative IDVs of FOSL2 was detected by western blot in GECs with linc00174 inhibition. Data represent mean ± SD (n = 3, each). **p < 0.01 versus linc00174(−)NC group. (C) Relative expression of FOSL2 mRNA was detected by quantitative real-time PCR in GECs after changing miR-138-5p. (D) Relative IDVs of FOSL2 was detected by western blot in GECs after changing miR-138-5p. Data represent mean ± SD (n = 3, each). *p < 0.05 and **p < 0.01 versus miR-138-5p(+)NC group; ^##p < 0.01 versus miR-138-5p(−)NC group. (E) Relative expression of FOSL2 mRNA was detected by quantitative real-time PCR in GECs after changing miR-150-5p. (F) Relative IDVs of FOSL2 was detected by western blot in GECs after changing miR-150-5p. Data represent mean ± SD (n = 3, each). *p < 0.05 and **p < 0.01 versus miR-150-5p(+)NC group; ^#p < 0.05 and ^##p < 0.01 versus miR-150-5p(−)NC group. (G) linc00174 co-transfection with miR-138-5p. Relative expression of FOSL2 mRNA was detected by quantitative real-time PCR. (H) linc00174 co-transfection with miR-138-5p. Relative IDVs of FOSL2 was detected by western blot. Data represent mean ± SD (n = 3, each). **p < 0.01 versus linc00174(−)NC + miR-138-5p(+)NC group. (I) linc00174 co-transfection with miR-150-5p. Relative expression of FOSL2 mRNA was detected by quantitative real-time PCR. (J) linc00174 co-transfection with miR-150-5p. Relative IDVs of FOSL2 was detected by western blot. Data represent mean ± SD (n = 3, each). **p < 0.01 versus linc00174(−)NC + miR-150-5p(+)NC group. (K) Relative luciferase activity was detected by Dual-Luciferase assay. Data represent mean ± SD (n = 3, each). **p < 0.01 versus FOSL2-3′ UTR-Wt+miR-138-5p(+)NC group. (L) Relative luciferase activity was detected by Dual-Luciferase assay. Data represent mean ± SD (n = 5, each). *p < 0.05 versus FOSL2-3′ UTR-Wt+miR-150-5p(+)NC group.