Table 1.
Primer or Probe | Gene | Sequence (5′→3′) or Assay ID |
---|---|---|
Primer | linc00174 | F: TGTGTCTACAGGCCCAACTG |
R: AAGGTGGGCCTAGCAAAGTT | ||
FOSL2 | F: GGGTAGATATGCCTGGCTCA | |
R: GGTATGGGTTGGACATGGAG | ||
GAPDH | F: AAATCCCATCACCATCTTCCAG | |
R: TGATGACCCTTTTGGCTCCC | ||
Probe | miR-138-5p | 2452336 (Applied Biosystems) |
miR-150-5p | 2824926 (Applied Biosystems) | |
U6 | 2819045 (Applied Biosystems) |
One-Step SYBR PrimeScript RT-PCR cycling conditions were as follows: 5 min at 42°C, 10 s at 95°C, 40 cycles of 3 s at 95°C, and 30 s at 60°C. The reverse transcription was set as follows: 30 min at 16°C, 30 min at 42°C, and 5 min at 85°C. PCR conditions were set as follows: 2 min at 50°C, 10 min at 95°C, 40 cycles of 15 s at 95°C, and 1 min at 60°C. F, forward; R, reverse.