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. 2019 Nov 9;18:1072–1090. doi: 10.1016/j.omtn.2019.10.031

Table 1.

Primers and Probes Used for Quantitative Real-Time PCR

Primer or Probe Gene Sequence (5′→3′) or Assay ID
Primer linc00174 F: TGTGTCTACAGGCCCAACTG
R: AAGGTGGGCCTAGCAAAGTT
FOSL2 F: GGGTAGATATGCCTGGCTCA
R: GGTATGGGTTGGACATGGAG
GAPDH F: AAATCCCATCACCATCTTCCAG
R: TGATGACCCTTTTGGCTCCC
Probe miR-138-5p 2452336 (Applied Biosystems)
miR-150-5p 2824926 (Applied Biosystems)
U6 2819045 (Applied Biosystems)

One-Step SYBR PrimeScript RT-PCR cycling conditions were as follows: 5 min at 42°C, 10 s at 95°C, 40 cycles of 3 s at 95°C, and 30 s at 60°C. The reverse transcription was set as follows: 30 min at 16°C, 30 min at 42°C, and 5 min at 85°C. PCR conditions were set as follows: 2 min at 50°C, 10 min at 95°C, 40 cycles of 15 s at 95°C, and 1 min at 60°C. F, forward; R, reverse.