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Crispr-cas12/cpf1-mediated genome-editing of counter-selectable marker orotidine 5′-phosphate decarboxylase encoded by URA3. (A) On-target genome-editing efficiency for URA3 with different crRNA configurations and modifications. N = 3, the reported data represents mean ± sd. (B) Indel mutations of URA3 genetic marker when the crRNA-URA3A targets to the sense strand. (C) Indel mutations of URA3 genetic marker when the crRNA-URA3B targets to the antisense strand.
