The change of copy numbers of overall bacteria (16S rRNA copies) and bacterial genera at different time points of the three queen groupings. UQ, unmated queen; MQ, mated queens; OQ, ovipositing queens. Days (d) for UQ represent days posteclosion and for MQ represent days postmating, with mating occurring at 7 days posteclosion. Bars represent means ± SEM. Different letters above bars within plots represent significant differences (pairwise t tests, P < 0.05).